Arabidopsis SABRE and CLASP interact to stabilize cell division plane orientation and planar polarity
نویسندگان
چکیده
The orientation of cell division and the coordination of cell polarity within the plane of the tissue layer (planar polarity) contribute to shape diverse multicellular organisms. The root of Arabidopsis thaliana displays regularly oriented cell divisions, cell elongation and planar polarity providing a plant model system to study these processes. Here we report that the SABRE protein, which shares similarity with proteins of unknown function throughout eukaryotes, has important roles in orienting cell division and planar polarity. SABRE localizes at the plasma membrane, endomembranes, mitotic spindle and cell plate. SABRE stabilizes the orientation of CLASP-labelled preprophase band microtubules predicting the cell division plane, and of cortical microtubules driving cell elongation. During planar polarity establishment, sabre is epistatic to clasp at directing polar membrane domains of Rho-of-plant GTPases. Our findings mechanistically link SABRE to CLASP-dependent microtubule organization, shedding new light on the function of SABRE-related proteins in eukaryotes.
منابع مشابه
RETRACTED: A PLETHORA-Auxin Transcription Module Controls Cell Division Plane Rotation through MAP65 and CLASP
Despite their pivotal role in plant development, control mechanisms for oriented cell divisions have remained elusive. Here, we describe how a precisely regulated cell division orientation switch in an Arabidopsis stem cell is controlled by upstream patterning factors. We show that the stem cell regulatory PLETHORA transcription factors induce division plane reorientation by local activation of...
متن کاملRetraction Notice to: A PLETHORA-Auxin Transcription Module Controls Cell Division Plane Rotation through MAP65 and CLASP
A PLETHORA-Auxin Transcription Module Controls Cell Division Plane Rotation through MAP65 and CLASP --Cell, Volume 149, Issue 2, 13 April 2012, Pages 383-396. Our paper reported that Arabidopsis PLETHORA transcription factors regulate cell division planes by transcriptional activation of MAP65, which interacts with the CLASP protein to guide microtubule orientation. We recently identified mista...
متن کاملThe Arabidopsis CLASP gene encodes a microtubule-associated protein involved in cell expansion and division.
Controlling microtubule dynamics and spatial organization is a fundamental requirement of eukaryotic cell function. Members of the ORBIT/MAST/CLASP family of microtubule-associated proteins associate with the plus ends of microtubules, where they promote the addition of tubulin subunits into attached kinetochore fibers during mitosis and stabilize microtubules in the vicinity of the plasma memb...
متن کاملCadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.
Oriented cell division is a fundamental determinant of tissue organization. Simple epithelia divide symmetrically in the plane of the monolayer to preserve organ structure during epithelial morphogenesis and tissue turnover. For this to occur, mitotic spindles must be stringently oriented in the Z-axis, thereby establishing the perpendicular division plane between daughter cells. Spatial cues a...
متن کاملAsymmetric cell division: Plane but not simple
The polarity of sensory bristles on the thorax of Drosophila is linked to the orientation of the asymmetric cell divisions that partition cell fate determinants in this lineage. The orientation of these divisions is under the control of the Frizzled pathway that generates planar polarity in a number of cell types.
متن کامل